human embryonic fibroblast cell line Search Results


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Labplus Inc human embryonic fibroblast cell line
Human Embryonic Fibroblast Cell Line, supplied by Labplus Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LGC Promochem mrc-5 human embryonal lung fibroblast cell line
Mrc 5 Human Embryonal Lung Fibroblast Cell Line, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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JCRB Cell Bank human embryonic fibroblast cell line nti-4
Human Embryonic Fibroblast Cell Line Nti 4, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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China Center for Type Culture Collection human fetal lung fibroblasts mrc5
Human Fetal Lung Fibroblasts Mrc5, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Eurobio human diploid embryonic lung fibroblasts mrc-5
Human Diploid Embryonic Lung Fibroblasts Mrc 5, supplied by Eurobio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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JCRB Cell Bank hf cells jcrb 1006.7
Cold stimulation induced cyclic AMP (cAMP) response element binding protein 1 (CREB1) phosphorylation in a frequency- and duration-dependent manner in vitro. In four cell lines of C2C12, 3T3-L1, human <t>fibroblast</t> (HF) and L6, cold stimulation induced CREB1 phosphorylation in a . phosphorylation, whereas two and three cold stimulations markedly induced CREB phosphorylation. The strongest and weakest CREB1 phosphorylation was apparent in the C2C12 and L6 cell lines, respectively. ( a ): C2C12 cells; ( b ): 3T3-L1 cells; ( c ): L6 cells; ( d ): HF cells. Con.: Control (no cold stimulation).
Hf Cells Jcrb 1006.7, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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JCRB Cell Bank normal human fibroblast cell line tig-1
Cold stimulation induced cyclic AMP (cAMP) response element binding protein 1 (CREB1) phosphorylation in a frequency- and duration-dependent manner in vitro. In four cell lines of C2C12, 3T3-L1, human <t>fibroblast</t> (HF) and L6, cold stimulation induced CREB1 phosphorylation in a . phosphorylation, whereas two and three cold stimulations markedly induced CREB phosphorylation. The strongest and weakest CREB1 phosphorylation was apparent in the C2C12 and L6 cell lines, respectively. ( a ): C2C12 cells; ( b ): 3T3-L1 cells; ( c ): L6 cells; ( d ): HF cells. Con.: Control (no cold stimulation).
Normal Human Fibroblast Cell Line Tig 1, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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China Center for Type Culture Collection human embryonic fibroblast cell line hfl1
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonic Fibroblast Cell Line Hfl1, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioResource International Inc human embryonic fibroblast kmst-6 cell line
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonic Fibroblast Kmst 6 Cell Line, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dainihon Jochugiku Co human embryonal diploid fibroblast cell line wi-38
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonal Diploid Fibroblast Cell Line Wi 38, supplied by Dainihon Jochugiku Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nissui Pharmaceutical human embryonic lung fibroblast cell line mrc-523
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonic Lung Fibroblast Cell Line Mrc 523, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Keygen Biotech human embryonic lung fibroblast cell line (helf)
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonic Lung Fibroblast Cell Line (Helf), supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cold stimulation induced cyclic AMP (cAMP) response element binding protein 1 (CREB1) phosphorylation in a frequency- and duration-dependent manner in vitro. In four cell lines of C2C12, 3T3-L1, human fibroblast (HF) and L6, cold stimulation induced CREB1 phosphorylation in a . phosphorylation, whereas two and three cold stimulations markedly induced CREB phosphorylation. The strongest and weakest CREB1 phosphorylation was apparent in the C2C12 and L6 cell lines, respectively. ( a ): C2C12 cells; ( b ): 3T3-L1 cells; ( c ): L6 cells; ( d ): HF cells. Con.: Control (no cold stimulation).

Journal: International Journal of Molecular Sciences

Article Title: Influence of Intermittent Cold Stimulations on CREB and Its Targeting Genes in Muscle: Investigations into Molecular Mechanisms of Local Cryotherapy

doi: 10.3390/ijms21134588

Figure Lengend Snippet: Cold stimulation induced cyclic AMP (cAMP) response element binding protein 1 (CREB1) phosphorylation in a frequency- and duration-dependent manner in vitro. In four cell lines of C2C12, 3T3-L1, human fibroblast (HF) and L6, cold stimulation induced CREB1 phosphorylation in a . phosphorylation, whereas two and three cold stimulations markedly induced CREB phosphorylation. The strongest and weakest CREB1 phosphorylation was apparent in the C2C12 and L6 cell lines, respectively. ( a ): C2C12 cells; ( b ): 3T3-L1 cells; ( c ): L6 cells; ( d ): HF cells. Con.: Control (no cold stimulation).

Article Snippet: These included mouse myoblasts (C2C12 cells: RCB0987, Riken BRC Cell Bank, Tsukuba, Ibaraki, Japan) mouse embryonic fibroblasts (3T3-L1 cells: JCRB9014, JCRB Cell Bank, Ibaraki, Osaka, Japan; original developers: Green et al.), human embryonic fibroblasts (HF cells: JCRB 1006.7, JCRB Cell Bank; original developers: Kouchi and Namba), rat skeletal muscle myoblasts (L6 cells: JCRB 9081, JCRB Cell Bank; original developer: Yaffe), and human embryonic kidney cells (HEK 293 cells; Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Binding Assay, Phospho-proteomics, In Vitro, Control

Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with HFL1 cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with HFL1 cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Derivative Assay, In Vitro, Incubation, Western Blot, Quantitative RT-PCR

SiO 2 ‐Exos induce lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) treated with various exosomes (SiO 2 ‐Exos or SiO 2 + GW4869‐Exos). Two‐way ANOVA; ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: SiO 2 ‐Exos induce lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) treated with various exosomes (SiO 2 ‐Exos or SiO 2 + GW4869‐Exos). Two‐way ANOVA; ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Migration, CCK-8 Assay, Wound Closure Assay

SiO 2 ‐Exo‐induced myofibroblast differentiation is ER stress dependent (A‐C, E) Western blot analysis of BIP, XBP1s, P ‐eIF2α, α‐SMA, Collagen I, and GAPDH in HFL1 cells (A, C) and NIH‐3T3 cells (B, E) incubated with SiO 2 ‐Exos (± 4‐PBA) at different points in time (0, 8, 16 or 24 h). (D, F) Analysis of collagen I, α‐SMA, and GAPDH in HFL1 cells (D) and NIH‐3T3 cells (F) incubated with SiO 2 ‐Exos or SiO 2 ‐Exos +4‐PBA for 24 h

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: SiO 2 ‐Exo‐induced myofibroblast differentiation is ER stress dependent (A‐C, E) Western blot analysis of BIP, XBP1s, P ‐eIF2α, α‐SMA, Collagen I, and GAPDH in HFL1 cells (A, C) and NIH‐3T3 cells (B, E) incubated with SiO 2 ‐Exos (± 4‐PBA) at different points in time (0, 8, 16 or 24 h). (D, F) Analysis of collagen I, α‐SMA, and GAPDH in HFL1 cells (D) and NIH‐3T3 cells (F) incubated with SiO 2 ‐Exos or SiO 2 ‐Exos +4‐PBA for 24 h

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Western Blot, Incubation

Inhibition of ER stress attenuates SiO 2 ‐Exo‐induced lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) incubated with SiO 2 ‐Exos ± 4‐PBA. Two‐way ANOVA; * P < .05, ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: Inhibition of ER stress attenuates SiO 2 ‐Exo‐induced lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) incubated with SiO 2 ‐Exos ± 4‐PBA. Two‐way ANOVA; * P < .05, ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Inhibition, Migration, CCK-8 Assay, Incubation, Wound Closure Assay