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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Influence of Intermittent Cold Stimulations on CREB and Its Targeting Genes in Muscle: Investigations into Molecular Mechanisms of Local Cryotherapy
doi: 10.3390/ijms21134588
Figure Lengend Snippet: Cold stimulation induced cyclic AMP (cAMP) response element binding protein 1 (CREB1) phosphorylation in a frequency- and duration-dependent manner in vitro. In four cell lines of C2C12, 3T3-L1, human fibroblast (HF) and L6, cold stimulation induced CREB1 phosphorylation in a . phosphorylation, whereas two and three cold stimulations markedly induced CREB phosphorylation. The strongest and weakest CREB1 phosphorylation was apparent in the C2C12 and L6 cell lines, respectively. ( a ): C2C12 cells; ( b ): 3T3-L1 cells; ( c ): L6 cells; ( d ): HF cells. Con.: Control (no cold stimulation).
Article Snippet: These included mouse myoblasts (C2C12 cells: RCB0987, Riken BRC Cell Bank, Tsukuba, Ibaraki, Japan) mouse embryonic fibroblasts (3T3-L1 cells: JCRB9014, JCRB Cell Bank, Ibaraki, Osaka, Japan; original developers: Green et al.),
Techniques: Binding Assay, Phospho-proteomics, In Vitro, Control
Journal: Journal of Cellular and Molecular Medicine
Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner
doi: 10.1111/jcmm.16524
Figure Lengend Snippet: Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with HFL1 cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and
Techniques: Derivative Assay, In Vitro, Incubation, Western Blot, Quantitative RT-PCR
Journal: Journal of Cellular and Molecular Medicine
Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner
doi: 10.1111/jcmm.16524
Figure Lengend Snippet: SiO 2 ‐Exos induce lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) treated with various exosomes (SiO 2 ‐Exos or SiO 2 + GW4869‐Exos). Two‐way ANOVA; ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant
Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and
Techniques: Migration, CCK-8 Assay, Wound Closure Assay
Journal: Journal of Cellular and Molecular Medicine
Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner
doi: 10.1111/jcmm.16524
Figure Lengend Snippet: SiO 2 ‐Exo‐induced myofibroblast differentiation is ER stress dependent (A‐C, E) Western blot analysis of BIP, XBP1s, P ‐eIF2α, α‐SMA, Collagen I, and GAPDH in HFL1 cells (A, C) and NIH‐3T3 cells (B, E) incubated with SiO 2 ‐Exos (± 4‐PBA) at different points in time (0, 8, 16 or 24 h). (D, F) Analysis of collagen I, α‐SMA, and GAPDH in HFL1 cells (D) and NIH‐3T3 cells (F) incubated with SiO 2 ‐Exos or SiO 2 ‐Exos +4‐PBA for 24 h
Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and
Techniques: Western Blot, Incubation
Journal: Journal of Cellular and Molecular Medicine
Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner
doi: 10.1111/jcmm.16524
Figure Lengend Snippet: Inhibition of ER stress attenuates SiO 2 ‐Exo‐induced lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) incubated with SiO 2 ‐Exos ± 4‐PBA. Two‐way ANOVA; * P < .05, ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant
Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and
Techniques: Inhibition, Migration, CCK-8 Assay, Incubation, Wound Closure Assay